Development of an ISFET based heparin sensor using the ion-step measuring method
نویسندگان
چکیده
The ion-step measuring method was used to determine heparin concentrations in PBS and blood plasma. Heparin is a sulphated polysaccharide which is clinically used as a drug to prevent the clotting of blood. The measuring method is based on detection of changes in charge density in a porous membrane which is deposited on the gate of an ISFET. Protamine was used as affinity ligand in the membrane. In PBS a linear relation was found between the heparin concentration and the ISFET response. The incubation time was reduced from 18 h to 15 min by increasing the porosity of the membrane. The results of the measurements in blood plasma show a significant nonspecific binding of plasma components in the membrane. Suggestions are given to prevent this nonspecific adsorption. The results described in this paper show a detection limit for the ion-step measuring method of at least 5 k lo-” Mel/l which is promising for future practical applications.
منابع مشابه
The ISFET based heparin sensor with a monolayer of protamine as affinity ligand.
The ion-step measuring method was used to determine absolute heparin concentrations in PBS and blood plasma with a Ta2O5 ISFET on to which a monolayer of protamine had been immobilized. Heparin is a highly negatively charged polysaccharide, which is used clinically to delay the clotting of blood. Protamine acts as an affinity ligand for heparin. The response of the ISFET system on a step-wise i...
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